Journal: Molecular cell

Article Title: Examining the roles of H3K4 methylation states with systematically characterized antibodies

doi: 10.1016/j.molcel.2018.08.015

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: RevMAb Cat#31-1039-00, RRID:AB_2716373 Anti-H3K4me3 antibody Thermo Fisher PA5-40086, Lot Number RL2301825.

Techniques: Recombinant, Synthesized, Peptide Microarray, Software

Journal: bioRxiv

Article Title: SAMD1 suppresses epithelial-mesenchymal transition (EMT) pathways in pancreatic ductal adenocarcinoma

doi: 10.1101/2023.08.14.553183

Figure Lengend Snippet: a) ChIP-qPCR at the CDH2 promoter with or without induction of SAMD1 rescue in PaTu8988t Control and SAMD1 KO cells using KDM1A and L3MBTL3 antibodies. Data represent the mean ± SD of two biological replicates. Significance was analyzed using Student’s t-test. b) ChIP-qPCR of CDH2 and L3MBTL3 promoter in PaTu8988t Control and SAMD1 KO cells using H3K4me2 and H3K4me3 antibodies. Data represent the mean ± SD of three biological replicates. Significance was analyzed using Student’s t-test. c) Western blot of an endogenous Co-IP between SAMD1 and KDM1A in PaTu8988t cells. d) Model of the interaction between SAMD1 and the KDM1A complex. e) Structure of SAMD1; co-immunoprecipitation in HEK293 cells showing the interaction between different SAMD1 deletion mutants and KDM1A. Regions identified to interact with KDM1A are marked red. f) Structure of KDM1A; co-immunoprecipitation in HEK293 cells showing the interaction between different KDM1A deletion mutants and SAMD1. Regions identified to interact with SAMD1 are marked red. g) Representative western blot of KDM1A IP in HEK293 cells, followed by histone demethylase assay. h) Quantification of four biological replicates of g). Significance was analyzed using one-way ANOVA.

Article Snippet: The following antibodies were used: a self-made SAMD1 antibody recognizing the SAM domain, a self-made L3MBTL3 antibody recognizing the SAM domain, anti-KDM1A (Abcam; AB17721), anti-H3K3me2 (Diagenode; C15410035), and anti-H3K4me3 (Diagenode; C15410003).

Techniques: Western Blot, Co-Immunoprecipitation Assay, Immunoprecipitation

Journal: bioRxiv

Article Title: SAMD1 suppresses epithelial-mesenchymal transition (EMT) pathways in pancreatic ductal adenocarcinoma

doi: 10.1101/2023.08.14.553183

Figure Lengend Snippet: a) Violin plots showing the SAMD1 level in PaTu8988t control and FBXO11 KO cells. Statistical significance was evaluated using a Kolmogorov-Smirnov test. b) SAMD1 levels in four different groups identified in in PaTu8988t control and FBXO11 KO cells. c) CpG island size of four different groups identified in . Statistical significance was evaluated using a Kolmogorov-Smirnov test. d) Heatmap of H3K4me3 and Pol II in the four different groups identified in . e) Profiles of H3K4me3 and RNA Polymer II at the four different groups identified in . f) Expression of genes at the four different groups identified in .

Article Snippet: The following antibodies were used: a self-made SAMD1 antibody recognizing the SAM domain, a self-made L3MBTL3 antibody recognizing the SAM domain, anti-KDM1A (Abcam; AB17721), anti-H3K3me2 (Diagenode; C15410035), and anti-H3K4me3 (Diagenode; C15410003).

Techniques: Expressing